Clathrin- and caveolae-independent entry of feline infectious peritonitis virus in monocytes depends on dynamin
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چکیده
منابع مشابه
Clathrin- and caveolae-independent entry of feline infectious peritonitis virus in monocytes depends on dynamin.
Feline infectious peritonitis virus (FIPV), a coronavirus that causes a lethal chronic disease in cats, enters feline monocytes via endocytosis. In this study, the pathway of internalization is characterized by evaluating the effect of chemical inhibitors and/or expression of dominant-negative (DN) proteins on the percentage of internalized virions per cell and infection. Further, co-localizati...
متن کاملSurface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway.
Infection with feline infectious peritonitis virus (FIPV), a feline coronavirus, frequently leads to death in spite of a strong humoral immune response. In previous work, we reported that infected monocytes, the in vivo target cells of FIPV, express viral proteins in their plasma membranes. These proteins are quickly internalized upon binding of antibodies. As the cell surface is cleared from v...
متن کاملFeline lectin activity is critical for the cellular entry of feline infectious peritonitis virus.
Feline infectious peritonitis is a lethal disease of felids caused by systemic infection with a feline coronavirus. Here, we report identification and analysis of the feline homologue to the human lectin DC-SIGN and show that it is a coreceptor for virulent strains of serotype 1 and serotype 2 feline coronaviruses.
متن کاملAttachment and internalization of feline infectious peritonitis virus in feline blood monocytes and Crandell feline kidney cells.
In this study, kinetics of attachment and internalization of feline infectious peritonitis virus (FIPV) serotype I strain Black and serotype II strain 79-1146 were determined in feline monocytes from two cats and in Crandell feline kidney (CrFK) cells. Attached FIPV I (Black) particles were observed on almost all monocytes. Within 1 h, 17 particles were bound per cell and, within 1 min, 89 % of...
متن کاملClathrin- and caveolin-1–independent endocytosis: entry of simian virus 40 into cells devoid of caveolae
imian Virus 40 (SV40) has been shown to enter host cells by caveolar endocytosis followed by transport via caveosomes to the endoplasmic reticulum (ER). Using a caveolin-1 (cav-1)–deficient cell line (human hepatoma 7) and embryonic fibroblasts from a cav-1 knockout mouse, we found that in the absence of caveolae, but also in wild-type embryonic fibroblasts, the virus exploits an alternative, c...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2008
ISSN: 0022-1317,1465-2099
DOI: 10.1099/vir.0.2008/001602-0